學術研究

緣起

穿透式電子顯微鏡(Transmission Electron Microscopy, TEM)具有高穿透能力及高解析度，為科學研究上最重要的工具之一。醫學院於97學年由宗倬章先生教育基金會捐款並結合理工與民生學院重點儀器設備費，設置宗倬章先生紀念電子顯微鏡實驗室，提供於臨床醫學、基礎醫學、生命科學、食品科學、材料科學與教學等相關方面服務和研究。

儀器設備

服務項目

組織固定、組織超薄切片、組織染色(正染色與負染色)及樣品的觀察與照相。

Cardiac muscle Hepatic Glomerulus Pancreas acinar cell

預約使用方法

  • 每人每次以預約一個單位(2小時/單位)為原則，且連續使用不得超過二個單位。若超過預約時間30分鐘仍未上機，本中心得將此時段安排給其他需要上機者。若發現實驗出問題無法上機，請親自來取消預約或打電話委託連絡人代為取消預約。
  • 預約方式：使用者於上班時間與管理員預約使用時間(聯絡人:吳彥陞 / 電話:(02)2905-2092)

樣品製備要求

  • 樣品請置於直徑3mm之銅網上
  • 不具揮發性
  • 無強磁性
  • 不含水分
  • 無腐蝕性

相關文件下載

 

研究成果

  1. Ramesh Kumar, B.Stephen Inbaraj, and B.H.Chen, (21 July 2010)Surface modification of superparamagnetic iron nanoparticles with calcium salt of poly(g-glutamicacid) as coating material. Materials Research Bulletin
  2. B Stephen Inbaraj, T H Kao, T Y Tsai, C P Chiu, R Kumar and B H Chen(14 January 2011) The synthesis and characterization of poly(γ -glutamic acid)-coated magnetite nanoparticles and their effects on antibacterial activity and cytotoxicity. Nanotechnology
  3. B.Stephen Inbaraj and B.H. Chen (June 24, 2011) Dye adsorption characteristics of magnetite nanoparticles coated with a biopolymer poly(r-glutamic acid). Bioresource Technology
  4. Yen-Yin Tsai, Yi-Huei Huang, Ya-Li Chao, Kuang-Yu Hu, Li-Te Chin, Shiu-Huey Chou, Ai-Ling Hour, Yeong-DerYao, Chi-Shun Tu, Yao-Jen Liang, Cheng-Yuh Tsai, Hao-YuWu, Shan-Wen Tan, and Han-Min Chen. (November 2, 2011) Identification of the Nanogold Particle-Induced Endoplasmic Reticulum Stress by Omic Techniques and Systems Biology Analysis.American Chemical Society NANO
  5. Baskaran Stephen Inbaraj and Bing-Huei Chen (9 August 2012) In vitro removal of toxic heavy metals by poly(γ-glutamic acid)-coated superparamagnetic nanoparticles. International Journal of Nanomedicine
  6. Kun-Long Hung, Su-Jane Wang, Ying-Chou Wang, Tsuey-Ru Chiang, Chia-Chuan Wang (29 October 2013) Upregulation of Presynaptic Proteins and Protein Kinases Associated with Enhanced Glutamate Release from Axonal Terminals (Synaptosomes) of the Medial Prefrontal Cortex in Rats with Neuropathic Pain. Pain
  7. C-H Lee, S-T Lin, J-J Liu, W-W Chang, J-L Hsieh and W-K Wang (23 January 2014) Salmonella induce autophagy in melanoma by the downregulation of AKT/mTOR pathway. Gene Therapy
  8. Yi Jyun Chen, Baskaran Stephen Inbaraj, Yeong Shiau Pu and Bing Huei Chen(20 March 2014) Development of lycopene micelle and lycopene chylomicron and a comparison of bioavailability.  Nanotechnology
  9. Fur-Jiang Leu, Chuen-Lin Huang, Yuh-Mou Sue, Shao-Chen Lee and Chia-Chen Wang(24 September 2014) Effects of tattoo ink’s absorption spectra and particle size on cosmetic tattoo treatment efficacy using Q-switched Nd:YAG laser. Lasers in Medical Science
  10. K Suresh Babu, M Anandkumar, TY Tsai, TH Kao, B Stephen Inbaraj and BH Chen (27 November 2014) Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles. International Journal of Nanomedicine
  11. Yesudoss Christu Rajan, Baskaran Stepene Inbaraj and Bing Huei Chen (21st January 2015) Synthesis and characterization of poly (r-glutamic acid)-based alumina nanoparticles with their protein adsorption efficiency and cytotoxicity towards human prostate cancer cells.The Royal Society of Chemistry
  12. Rwei-Fen S Hunag, Yi-Jun Wei, Baskaran Stephen Inbaraj and Bing-Huei Chen (8 April 2015) Inhibition of Colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene.International Journal of Nanomedicine
  13. Hong-Bin Chang and Bing Huei Chen (6 August 2015) Inhibition of lung cancer cells A549 and H460 by curcuminoid extracts and nanoemulsions prepared from Curcuma Ionga Linnaeus. Internation Journal of Nanomedicine
  14. Hong-Bin Chang and Bing Huei Chen (6 May 2016) Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3. Internation Journal of Nanomedicine
  15. Nai-Hsing Ho, Baskaran Stephene Inbaraj and Bing Huei Chen (6 May 2016) Utilization of Microemulisions from Rhinacanthus nasutus(L.) Kura to Improve Carotenoid Bioavailability. Scinetific Reports
  16. Tzu Yu Lin, Cheng Wei Lu, Su Jane Wang (13 May 2016)Luteolin protects the hippocampus against neuron impairments induced by kainic acid in rats. NeuroToxicology
  17. Tsung-Hsuan Lai, Ching-Hu Chung, Bing-Huei Chen, Chi-Feng Hung, Baskaran Stephen Inbaraj, Ming-Chieh Ma, Han-Min Chen, Chih-Jen Tsou, Pi-Hui Wu, and Wen-Bin Wu (3 April 2016) Gold Nanoparticles Compromise TNF-a Induced Endothelial Cell Adhesion Molecule Expression Through NF-kB and Protein Degradation Pathways and Reduce Neointima Formation in a Rat Carotid Balloon Injury Model. Biomedical Nanotechnology
  18. Hung-sheng Shang, Yi-No Wo, Chun-Hou Liao, Tzong-Shi Chiueh, Yuh-Feng Lin and Han-Sun Chiang (20 July 2016) Long-term administration of ketamine induces erectile dysfunction by decreasing neuronal nitric oxide synthase on cavernous nerve and increasing corporal smooth muscle cell apoptosis in rats. Oncotarget
  19. W D Lee, Y J Liang and B H Chen (11 Nov 2016) Effects of tanshinone nanoemulsion and extract on inhibition of lung cancer cells A549. Nanotechnology
  20. H J HSU, R F Huang, T H Kao, B S Inbaraj and B H Chen (7 march 2017) Preparation of carotenoid extracts and nanoemulsions from Lycium barbarum L. and their effects on growth of HT-29 colon cancer cells.Nanotechnology
  21. Ya-Yun Wang, Ying-Hung Lin, Yi-No Wu, Yen-Lin Cheng, Yung-Chih Lin, Chiao-Yin Cheng, Han-Sun Chiang (April 6 2017) Loss of SLC9A3 decreases CFTR protein and causes obstructed azoospermia in mice.PLOS GENETICS
  22. Ji-Fan Lin, Yi-Chia Lin, Te-Fu Tsai, Hung-En Chen, Kuang-Yu Chou and Thomas I-Sheng Hwang (16 May 2017) Cisplatin induces protective autophagy through activation of BECNI in human bladder cancer cells. Drug Design, Development and Therapy
  23. Pei Shan Lu, Baskaran Stephen Inbaraj and Bing Huei Chen (18 May 2017) Determination of oral bioavailability of curcuminoid dispersions and nanoemulsions prepared from Curcuma longa Linnaeus.   J Sci Food Argic
  24. Chu-Ching Yang, Chi-Feng Hung and Bing-Huei Chen (5 Sep 2017) Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth. International Journal of Nanomedicine
  25. Yi-No Wu, Kuo-Chiang Chen, Chun-Hou Liao and Han-Sun Chiang (16 October 2017) Spontaneous Regeneration of Nerve Fiber and Irreversibility of Corporal Smooth Muscle Fibrosis after Cavernous Nerve Crush Injury: Evidence From Serial Transmission Electron Microscopy and Intracavernous Pressure.Urology
  26. Ya-Yun Wang, Han-Sun Chiang, Chian-Tin Cheng, Yi-No Wu, Yung-Chih Lin, Hsuan-Che Liu, Wei-Kung Tsai, Yen-Lin Chen and Ying-Hung Lin (29 Dec 2017) SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells. International Journal of Molecular Sciences
  27. YihHuei Uen, Jin-Wun Wang, ChiaChen Wang, Yaoyun Jhang, Jo-Yun Chung, Tin Ting Tseng, MingJen Sheu and ShaoChen Lee (June 15, 2018) Mining of potential microRNAs with clinical correlation – regulation of syndecan-1 expression by miR-122-5p altered mobility of breast cancer cells and possible correlation with liver injury. Oncotarget
  28. Wei-Jung Chang, Bing-Huei Chen, Baskaran Stephen Inbaraj and John-Tung Chien (25 Jan 2019) Preparation of allyl isothiocyanate nanoparticles, their anti-infammatory activity towards RAW 264.7 macrophage cells and anti-proliferative effect on HT1376 bladder cancer cells.  Society of Chemical Industry
  29. Ya-Yun Wan, Tsung-Hsuan Lai, Mei-Feng Chen, Hui-Ling Lee, Pao-Lin Kuo and Ying-Hung Lin (23 Aug 2019) SEPT14 Mutation and Teratozoospermia: Genetic Effects on Sperm Head Morphology and DNA Integrity.  Journal of Clinical Medicine
  30. Yi-No Wu, Kuo-Chiang Chen, Chun-Hou Liao, Chien Liang Liu and Han-Sun Chiang (16 Nov 2019) Smooth Muscle Progenitor Cells Preserve the Erectile Function by Reducing Corporal Smooth Muscle Cell Apoptosis after Bilateral Cavernous Nerve Crush Injury in Rats.  Biomed Research International
  31. Ming-Chang Chiang, Christopher J. B. Nicol, Yi-Chuan Cheng, Chiahui Yen, Chien-Hung Lin, Shiang-Jiuun Chen and Rong-Nan Huang (21 May 2020) Nanoglod Neuroprotection in Human Neural Stem Cells Against Amyloid-beta-induced Mitrochondrial Dysfunction. Neuroscience
  32. Baskaran Stephen Inbaraj , Bang-Yuan Chen , Chia-Wei Liao, Bing-Huei Chen (07 Aug 2020)Green synthesis, characterization and evaluation of catalytic and antibacterial activities of chitosan, glycol chitosan and poly(γ-glutamic acid) capped gold nanoparticles.International Journal of Biological Macromolecules
  33. Ya-Yun Wang, Chih-Chun Ke, Yen-Lin Chen, Yu-Hua Lin, I-Shing Yu, Wei-Chi Ku, Moira K. O’Bryan, Ying-Hung Lin (25 Sep 2020) Deficiency of the Tbc1d21 gene causes male infertility with morphological abnormalities of the sperm mitochondria and flagellum in mice.  PLOS GENETICS
  34. Chin-Hsien Lin, Pei-I Tsai, Han-Yi Lin, Nobutaka Hattori, Manabu Funayama, Beomseok Jeon, Kota Sato, Koji Abe, Yohei Mukai, Yuji Takahashi, Yuanzhe Li, Kenya Nishioka, Hiroyo Yoshino, Kensuke Daida, Meng-Ling Chen, Jay Cheng, Cheng-Yen Huang, Shiou-Ru Tzeng, Yen-Sheng Wu, Hsing-Jung Lai, Hsin-Hsi Tsai, Ruoh-Fang Yen, Ni-Chung Lee, Wen-Chun Lo, Yu-Chien Hung, Chih-Chiang Chan, Yi-Ci Ke, Chi-Chao Chao, Sung-Tsang Hsieh, Matthew Farrer, Ruey-Meei Wu (5 Dec 2020) Mitochondrial UQCRC1 mutations cause autosomal dominant parkinsonism with polyneuropathy.  Brain
  35. Yi-No Wu, Chun-Hou Liao, Kuo-Chiang Chen, Han-Sun Chiang (21 January 2021) Dual effect of chitosan activated platelet rich plasma (cPRP) improved efectile function after carernous nerve injury.  journal of the Formosan Medical Association
  36. Baskaran Stephen Inbaraj, Kandi Sridhar, Bing-Huei Chen (17 March 2021) Removal of polycyclic aromatic hydrocarbons from water by magnetic activated carbon nanocomposite from green tea waste.  Journal of Hazardous Materials
  37. Hsun-Hao Chang, Horng-Huey Ko, Tzy-Ming Lu, Jia-Ying Lin, Der-Chen Chang, Thomas W. Chu and Chi-Feng Hung (21 March 2021) Inhibition of UVA Damage on Human Skin Dermis Fibroblasts by the Isoflavonoid Intermediate Deoxybenzoin-3A.  Chemical Research in Toxicology
  38. Fur-Jiang Leu, Chuen-Lin Huang, Yen-Sheng Wu, Chia-Chen Wang (9 August 2021) Comparison of picosecond versus nanosecond Nd: YAG lasers for the removal of cosmetic tattoos in an animal model.  Lasers in Medical Science
  39. Man-Hai Liu, Yi-Fen Li and Bing-Huei Chen (12 August 2021) Preparation of Chlorophyll Nanoemulsion from Pomelo Leaves and Its Inhibition Effect on Melanoma Cell A375.   Plants
  40. Kuan-Ming Chiu, Yen-Ling Hung, Su-Jane Wang, Yi-Ju Tsai, Nan-Lin Wu, Cher-Wei Liang, Der-Chen Chang and Chi-Feng Hung (12 October 2021)  Anti-allergic and Anti-Inflammatory effects of Neferine on RBL-2H3 cells.  Molecular Sciences
  41. Chun-Yu Chen, Tsung-Yu Tsai and Bing-Huei Chen (13 October 2021)Effects of Black Garlic Extract and Nanoemulsion on the Deoxy Corticosterone Acetate-Salt Induced Hypertension and Its Associated Mild Cognitive Impairment in Rats. Antioxidants
  42. Baskaran Stephen Inbaraj, Leng-Huei Hua and Bing-Huei Chen (29 October 2021) Comparative Study on Inhibition of Pancreatic Cancer Cells by Resveratrol Gold Nanoparticles and a Resveratrol Nanoemulsion Prepared from Grape Skin. Pharmaceutics
  43. Chun-Hou Liao, Kau-Han Lee, Shiu-Dong Chung, Kuo-Chiang Chen, Chellappan Praveen Rajneesh, Bo-He Chen, Jai-Hong Cheng, Wang-Ying Lin, Han-Sun Chiang and Yi-No Wu (9 March 2022) Intracavernous Injection of Platelet-Rich Plasma Therapy Enhances Efectile Function and Decreases the Mortality Rate in Streptozotocin-Induced Diabetic Rats.   Internation Journal of Molecular Sciences
  44. Shiu-Dong Chung, Chellappan Praveen Rajneesh, Kuo-Chiang Chen, Huai-Ching Tai, Meng-Lin Chang, Xiao-Wen Tseng, Jai-Hong Cheng, Wei-Kung Tsai, Han-Sun Chiang and Yi-No Wu (14 February 2022) Specific Impacts of Ketamine on Bladder Dysfunction and Associated Histological in Rats-A Time Coerse Validation through Transmission Electron Microscopy.  Internation Journal of Molecular Sciences
  45. Kandi Sridhar, Baskaran Stephene Inbaraj, Bing-Huei Chen (25 April 2022) An improved surface enhanced Raman spectroscopic method using a paper-based grape skin-gold nanoparticles/graphene oxide substrate for detection of rhodamine 6G in water and food. Chemosphere
  46. Hsin-Yen Hsu and Bing-Huei Chen (2 May 200) A Comparative Study on Inhibition of Breast Cancer Cells and Tumors in Mice by Carotenoid Extract and Nanoemulsion Prepared from Sweet Potato (Ipomoea batatas L. Peel) pharmaceutics
  47. Chen-Te Jen, Bo-Yang Hsu, Bing-Huei Chen(18 october 2022) A study on anti-fatigue effects in rats by nanoemulsion and liposome prepared from American ginseng root residue extract.  Food Bioscience
  48. Yu-Chi-Huang and Bing-Huei Chen (23 Dec 2022) A comparative study on Improving streptozotocin-induce type-2 diabetes in rats by hydrosol, extract and nanoemulsion prepared from cinnamon leaves.  Antioxidants
  49. Hsin-Rong Yu and Bing-Huei Chen (10 May 2023) Analysis of phenolic acids and flavonoids in rabbiteye blueberry leaves by UPLC-MS/MS and preparation of nanoemulsions and extracts for improving antiaging effects in mice.  foods
  50. Hsin-Rong Yu and Bing-Huei Chen (10 May 2023) Analysis of phenolic acids and flavonoids in rabbiteye blueberry leaves by UPLC-MS/MS and preparation of nanoemulsions and extracts for improving antiaging effects in mice.  Foods
  51. Yi Chun Wang, Vinchi Wang and Bing Huei Chen (02 Aug 2023) Analysis of bioactive compounds in cinnamon leaves and preparation of nanoemulsion and byproducts for improving Parkinson’s diin rats.  Frontiers in Nutrition
  52. Nian-Ting Hou and Bing-Huei Chen (12 Sep 2023) Preparation of Nanoemulsions with Low-Molecular-Weight Collagen Peptides from Sturgeno Fish Skin and Evaluation of Anti-Diabetic Wound-Healing Effects in Mice.  Pharmaceutics

實驗室地點與聯繫方式

  • 地點：輔仁大學醫學院國璽樓MD313
  • 聯絡人：吳彥陞 先生
  • 電話：(02)2905-2092
  • 送樣時間：9:00-17:00 (週一至週五)
  • 使用者請於上班時間與技術員聯絡詢問儀器使用狀況。