服務項目
- AmpliSeq_Exome
- AmpliSeq_Cancer hotspot panels
- AmpliSeq_custom panels
- 16S metagenomics
- PCR-Free whole genome DNA sequencing
- RNA-Seq
- Oncomine Cancer Research Panels
樣品送件要求
服務項目 | DNA需求 | |||
total amount | concentration | 260/280 | 260/230 | |
AmpliSeq_Exome | >200ng | >5ng/ul | _ | _ |
AmpliSeq_Cancer hotspot panels | >20ng | >2ng/ul | _ | _ |
AmpliSeq_custom panels* | >50ng | >5ng/ul | _ | _ |
16S metagenomics | >10ng | >2ng/ul | >1.8 | >1.7 |
PCR-Free whole genome DNA sequencing | >2ug | >50ng/ul | >1.8 | >1.7 |
RNA-Seq | _ | _ | _ | _ |
Oncomine Cancer Research Panels | >40ng | >3.33ng/ul | _ | _ |
服務項目 | RNA需求 | ||||
total amount | concentration | 260/280 | 260/230 | RIN | |
AmpliSeq_Exome | _ | _ | _ | _ | _ |
AmpliSeq_Cancer hotspot panels | _ | _ | _ | _ | _ |
AmpliSeq_custom panels* | >50ng | >100ng/ul | >1.8 | >1.7 | >8 |
16S metagenomics | _ | _ | _ | _ | _ |
PCR-Free whole genome DNA sequencing | _ | _ | _ | _ | _ |
RNA-Seq | >5ng | >100ng/ul | >1.8 | >1.7 | >8 |
Oncomine Cancer Research Panels | >20ng | >1.4ng/ul | _ | _ | _ |
收費標準
- Library Construction :
Type |
校內 (含教學醫院、建教合作單位) |
校 外 |
DNA | 6000 | 10000 |
RAN | 6000 | 10000 |
Ampliseq (1 pool) | 6000 | 10000 |
Ampliseq (2 pool) | 10000 |
- Sequencing :
Chip | Reads | Max Read Length |
校內
(教學醫院、建教合作單位)
|
校外 |
314Chip | 0.4M | 400 | 20000 | 25000 |
316Chip | 2M | 400 | 28000 | 35000 |
318Chip | 4M | 400 | 35000 | 45000 |
- Bioinformatics :
- Example :
Sample number: 10
DNA Library construction: 校內$6,000、校外$10,000Subtotal: 校內$60,000、校外$100,000
Sequencing: 314 Chip (on average 40,000 reads/sample)
Chip number: 1
Subtotal: 校內$20,000、校外$25,000
Bioinformatics:
校內(60,000+20,000)x10%= $8,000
校外(100,000+25,000)x10%= $12,500)
Total cost: 校內$88,000、校外$137,500
樣品送件須知
- DNA:Genomic DNA、ChIP DNA、PCR amplicon
- RNA:mRNA、small RNA
- 請將樣品回溶於Nuclease free water
- 送件前,樣品品質、濃度、總量的部分需要符合定序服務樣品需求
- 收件後,實驗室會再次確認樣品品質是否符合需求,確定後會進行通知樣品是否合格,QC未過,但送件者基於實驗需求,仍欲進行製備,請簽署同意書以繼續進行實驗
實驗室地點與聯繫方式
- 地點:輔仁大學醫學院國璽樓MD312
- 聯絡人:林麗如 小姐
- 電話:(02)2905-6391
- 送樣時間:9:00-17:00 (週一至週五)
- 使用者請於上班時間與技術員聯絡詢問儀器使用狀況。